Recombinant humaan GTF2A1-eiwit

TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity. Recombinant human GTF2A1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Transcription initiation factor IIA subunit 1 isoform 1, General transcription factor IIA, 1, 19/37kDa, TF2A1, TFIIA, TFIIA-42, TFIIAL

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

32.2 kDa (297aa) confirmed by MALDI-TOF

GTF2A1, ATGP2471-10 µg, ATGP2471-20 µg, ATGP2471-50 µg, ATGP2471-100 µg, ATGP2471-250 µg, ATGP2471-500 µg, ATGP2471-1 mg, ATGP2471-10, ATGP2471-20, ATGP2471-50, ATGP2471-100, ATGP2471-250, ATGP2471-500, ATGP2471-1

Zhou H., Spicuglia S et al. (2006), Mol. Cell. Biol. 26:2728-2735

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.15M NaCl, 1mM DTT

Epigenomics (Transcription & Translation)

NP_056943

P52655

Human

MANSANT NTVPKLYRSV IEDVINDVRD IFLDDGVDEQ VLMELKTLWE NKLMQSRAVD GFHSEEQQLL LQVQQQHQPQ QQQHHHHHHH QQAQPQQTVP QQAQTQQVLI PASQQATAPQ VIVPDSKLIQ HMNASNMSAA ATAATLALPA GVTPVQQILT NSGQLLQVVR AANGAQYIFQ PQQSVVLQQQ VIPQMQPGGV QAPVIQQVLA PLPGGISPQT GVIIQPQQIL FTGNKTQVIP TTVAAPTPAQ AQITATGQQQ PQAQPAQTQA PLVLQVD