Recombinant humaan GTF2E2-eiwit

GTF2E2, also known as TFIIE, is essential for transcription initiation by RNA polymerase II in conjunction with other general factors. TFIIE is a heterotetramer containing two subunits of relative molecular mass 57, 000 (TFIIE-alpha) and two of 34, 000 (TFIIE-beta) . TFIIE-beta is required in conjunction with TFIIE-alpha for transcription initiation. GTF2E2 recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase. Recombinant human GTF2E2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Transcription initiation factor IIE subunit beta, FE, TF2E2, TFIIE-B

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

35.6 kDa (315aa) confirmed by MALDI-TOF

GTF2E2, Transcription initiation factor IIE subunit beta, FE, TF2E2, TFIIE-B, ATGP1547-10 µg, ATGP1547-20 µg, ATGP1547-50 µg, ATGP1547-100 µg, ATGP1547-250 µg, ATGP1547-500 µg, ATGP1547-1 mg, ATGP1547-10, ATGP1547-20, ATGP1547-50, ATGP1547-100, ATGP1547-250, ATGP1547-500, ATGP1547-1

Kang S.-W., et al. (2000) Genes Cells. 5:251-263; ; Liu L., et al. (2009) J. Biol. Chem. 284:5165-5174

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.1M NaCl, 1mM DTT

Epigenomics (Transcription & Translation)

NP_002086

P29084

Human

MDPSLL RERELFKKRA LSTPVVEKRS ASSESSSSSS KKKKTKVEHG GSSGSKQNSD HSNGSFNLKA LSGSSGYKFG VLAKIVNYMK TRHQRGDTHP LTLDEILDET QHLDIGLKQK QWLMTEALVN NPKIEVIDGK YAFKPKYNVR DKKALLRLLD QHDQRGLGGI LLEDIEEALP NSQKAVKALG DQILFVNRPD KKKILFFNDK SCQFSVDEEF QKLWRSVTVD SMDEEKIEEY LKRQGISSMQ ESGPKKVAPI QRRKKPASQK KRRFKTHNEH LAGVLKDYSD ITSSK