Recombinant humaan GTF2F2-eiwit

GTF2F2, also known as TFIIF, is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA-helicase activity. Recombinant human GTF2F2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

General transcription factor IIF subunit 2, BTF4; RAP30; TF2F2; TFIIF

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

30.5 kDa (269aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

GTF2F2, General transcription factor IIF subunit 2, BTF4; RAP30; TF2F2; TFIIF, ATGP1524-10 µg, ATGP1524-20 µg, ATGP1524-50 µg, ATGP1524-100 µg, ATGP1524-250 µg, ATGP1524-500 µg, ATGP1524-1 mg, ATGP1524-10, ATGP1524-20, ATGP1524-50, ATGP1524-100, ATGP1524-250, ATGP1524-500, ATGP1524-1

Purrello M. et al. . (1995) Cytogenet Cell Genet. 69 (1-2) :75-80.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 0.2M NaCl, 1mM DTT

Epigenomics (Transcription & Translation)

NP_004119.1

P13984

Human

MAERGELDLT GAKQNTGVWL VKVPKYLSQQ WAKASGRGEV GKLRIAKTQG RTEVSFTLNE DLANIHDIGG KPASVSAPRE HPFVLQSVGG QTLTVFTESS SDKLSLEGIV VQRAECRPAA SENYMRLKRL QIEESSKPVR LSQQLDKVVT TNYKPVANHQ YNIEYERKKK EDGKRARADK QHVLDMLFSA FEKHQYYNLK DLVDITKQPV VYLKEILKEI GVQNVKGIHK NTWELKPEYR HYQGEEKSD