Recombinant humaan GTF3C6-eiwit

RNA polymerases are unable to initiate RNA synthesis in the absence of additional proteins called general transcription factors (GTFs) . GTFs assemble in a complex on the DNA promoter and recruit the RNA polymerase. GTF3C family proteins (e. g., GTF3C1, MIM 603246) are essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA (MIM 180420), tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. Recombinant human GTF3C6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

General transcription factor 3C polypeptide 6, General transcription factor 3C polypeptide 6, bA397G5.3, C6orf51, TFIIIC35

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

26.4 kDa (236aa) confirmed by MALDI-TOF

GTF3C6, General transcription factor 3C polypeptide 6, General transcription factor 3C polypeptide 6, bA397G5.3, C6orf51, TFIIIC35, ATGP2885-10 µg, ATGP2885-20 µg, ATGP2885-50 µg, ATGP2885-100 µg, ATGP2885-250 µg, ATGP2885-500 µg, ATGP2885-1 mg, ATGP2885-10, ATGP2885-20, ATGP2885-50, ATGP2885-100, ATGP2885-250, ATGP2885-500, ATGP2885-1

Dumay-Odelot, H., et al. (2007) J. Biol. Chem. 282 (23), 17179-17189

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_612417

Q969F1

Human

MGSSHHHHHH SSGLVPRGSH MGSMAAAADE RSPEDGEDEE EEEQLVLVEL SGIIDSDFLS KCENKCKVLG IDTERPILQV DSCVFAGEYE DTLGTCVIFE ENVEHADTEG NNKTVLKYKC HTMKKLSMTR TLLTEKKEGE ENIGGVEWLQ IKDNDFSYRP NMICNFLHEN EDEEVVASAP DKSLELEEEE IQMNDSSNLS CEQEKPMHLE IEDSGPLIDI PSETEGSVFM ETQMLP