Recombinant humaan Guanine deaminase/GDA-eiwit

GDA is an enzyme responsible for the hydrolytic deamination of guanine. Studies in rat ortholog suggest this gene plays a role in microtubule assembly. Multiple transcript variants encoding different isoforms have been found for this gene. Recombinant human GDA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is >2,000 pmol/min/ug, and is defined as the amount of enzyme that convert guanine to xanthine per minute at pH 8.0 at 37C.

Guanine deaminase isoform b, Guanase, Guanine aminase, Guanine aminohydrolase, GAH, p51-nedasin, CYPIN, Cytoplasmic PSD-95 interactor

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

Specific activity is >2,000pmol/min/ug, and is defined as the amount of enzyme that convert guanine to xanthine per minute at pH 8.0 at 37C.

53 kDa (477aa)

GDA, Guanine deaminase, CYPIN, GUANASE, NEDASIN, ATGP3429-10 µg, ATGP3429-20 µg, ATGP3429-50 µg, ATGP3429-100 µg, ATGP3429-250 µg, ATGP3429-500 µg, ATGP3429-1 mg, ATGP3429-10, ATGP3429-20, ATGP3429-50, ATGP3429-100, ATGP3429-250, ATGP3429-500, ATGP3429-1

Gang Yuan, James C. Bin, et al. (1999) . J. Biol. Chem. 274 (12) :8175-80.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_004284

Q9Y2T3

Human

MGSSHHHHHH SSGLVPRGSH MGSMCAAQMP PLAHIFRGTF VHSTWTCPME VLRDHLLGVS DSGKIVFLEE ASQQEKLAKE WCFKPCEIRE LSHHEFFMPG LVDTHIHASQ YSFAGSSIDL PLLEWLTKYT FPAEHRFQNI DFAEEVYTRV VRRTLKNGTT TACYFATIHT DSSLLLADIT DKFGQRAFVG KVCMDLNDTF PEYKETTEES IKETERFVSE MLQKNYSRVK PIVTPRFSLS CSETLMGELG NIAKTRDLHI QSHISENRDE VEAVKNLYPS YKNYTSVYDK NNLLTNKTVM AHGCYLSAEE LNVFHERGAS IAHCPNSNLS LSSGFLNVLE VLKHEVKIGL GTDVAGGYSY SMLDAIRRAV MVSNILLINK VNEKSLTLKE VFRLATLGGS QALGLDGEIG NFEVGKEFDA ILINPKASDS PIDLFYGDFF GDISEAVIQK FLYLGDDRNI EEVYVGGKQV VPFSSSV