Recombinant humaan H-Ras-eiwit

HRAS, also known as GTPase HRas, belongs to the Ras family which function in signal transduction pathways. This protein can bind GTP and GDP, and it has intrinsic GTPase activity. HRAS undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. It also may play a role in regulating the kinetics of signaling in the phototransduction cascade. Defects in HRAS are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Recombinant human HRAS protein was expressed in E. coli and purified by using conventional chromatography techniques.

GTPase Hras, HRas proto-oncogene GTPase, HRAS, HRAS1, v-Ha-ras Harvey rat sarcoma viral oncogene homolog, Harvey rat sarcoma viral oncogene homolog, H-Ras-1, Ha-Ras, Transforming protein p21, c-H-ras, p21ras

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

22.0 kDa (194aa) confirmed by MALDI-TOF

HRAS, v-Ha-ras Harvey rat sarcoma viral oncogene homolog, HMG14, GC104230, v-Ha-ras Harvey rat sarcoma viral oncogene homolog C BAS/HAS, c H ras, C HA RAS1, c has/bas p21 protein, c ras Ki 2 activated oncogene, CTLO, GTP and GDP binding peptide B, H Ras 1, H RASIDX, Ha Ras, Ha Ras1 proto oncoprotein, HAMSV, HRAS, HRAS1, K ras, N ras, p19 H RasIDX protein, p21ras, Ras family small GTP binding protein H Ras, RASH1, Transformation gene oncogene HAMSV, Transforming protein p21, v Ha ras Harvey rat sarcoma viral oncogene homolog, VH Ras, vHa RAS., ATGP0516-10 µg, ATGP0516-20 µg, ATGP0516-50 µg, ATGP0516-100 µg, ATGP0516-250 µg, ATGP0516-500 µg, ATGP0516-1 mg, ATGP0516-10, ATGP0516-20, ATGP0516-50, ATGP0516-100, ATGP0516-250, ATGP0516-500, ATGP0516-1

McCormick F. (1995) Mol Reprod Dev. 42 (4) :500-6; ; Ayllon V. et al. (2000) Mol Membr Biol. 17 (2) :65-73;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl

Signal Transduction

NP_001123914.1

P01112

Human