Recombinant humaan H2AFZ-eiwit

Histone H2A. Z, also known as H2AFZ, is a member of the histone H2A family. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . H2AFZ may be involved in the formation of constitutive heterochromatin and may be required for chromosome segregation during cell division. Also, H2AFZ is a variant Histone H2A which replaces conventional H2A in a subset of nucleosomes. Recombinant human H2AFZ protein, fused to His-tag at N-terminus, was expressed in E. coli.

Histone H2A.Z, H2A.z, H2A/z, H2AZ

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

15.9 kDa (151aa)

H2AFZ, Histone H2A.Z, H2A.z, H2A/z, H2AZ, ATGP2612-10 µg, ATGP2612-20 µg, ATGP2612-50 µg, ATGP2612-100 µg, ATGP2612-250 µg, ATGP2612-500 µg, ATGP2612-1 mg, ATGP2612-10, ATGP2612-20, ATGP2612-50, ATGP2612-100, ATGP2612-250, ATGP2612-500, ATGP2612-1

Rupp R A., et al. (2005) Cell. 123: 1178-1179.; ; Martin C., et al. (2005) Mol Cell Biol. 6:838-849.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Epigenomics (Transcription & Translation)

NP_002097

P0C0S5

Human

MAGGKAG KDSGKAKTKA VSRSQRAGLQ FPVGRIHRHL KSRTTSHGRV GATAAVYSAA ILEYLTAEVL ELAGNASKDL KVKRITPRHL QLAIRGDEEL DSLIKATIAG GGVIPHIHKS LIGKKGQQKT V