Recombinant humaan HAAO-eiwit

HAAO is a monomeric cytosolic protein belonging to the family of intramolecular dioxygenases containing nonheme ferrous iron. This protein catalyzes the synthesis of quinolinic acid (QuIN) from 3-hydroxyanthranilic acid. QuIN is an excitotoxin whose toxicity is mediated by its ability to activate glutamate N-methyl-D-aspartate receptors. Increased cerebral levels of QuIN may participate in the pathogenesis of neurologic and inflammatory disorders. HAAO has been suggested to play a role in disorders associated with altered tissue levels of QuIN. Recombinant human HAAO protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

3-hydroxyanthranilate 34-dioxygenase, 3-hydroxyanthranilate 3,4-dioxygenase, 3-HAO, HAO

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by BCA assay)

> 90% by SDS-PAGE

35 kDa (310aa) confirmed by MALDI-TOF

HAA, 3-hydroxyanthranilate 34-dioxygenase, 3-hydroxyanthranilate 3,4-dioxygenase, 3-HAO, HAO, ATGP1559-10 µg, ATGP1559-20 µg, ATGP1559-50 µg, ATGP1559-100 µg, ATGP1559-250 µg, ATGP1559-500 µg, ATGP1559-1 mg, ATGP1559-10, ATGP1559-20, ATGP1559-50, ATGP1559-100, ATGP1559-250, ATGP1559-500, ATGP1559-1

Malherbe P., et al. (1994) J Biol Chem. 13; ;269 (19) :13792-7.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Metabolism

NP_036337

P46952

Human

MERRLG VRAWVKENRG SFQPPVCNKL MHQEQLKVMF IGGPNTRKDY HIEEGEEVFY QLEGDMVLRV LEQGKHRDVV IRQGEIFLLP ARVPHSPQRF ANTVGLVVER RRLETELDGL RYYVGDTMDV LFEKWFYCKD LGTQLAPIIQ EFFSSEQYRT GKPIPDQLLK EPPFPLSTRS IMEPMSLDAW LDSHHRELQA GTPLSLFGDT YETQVIAYGQ GSSEGLRQNV DVWLWQLEGS SVVTMGGRRL SLAPDDSLLV LAGTSYAWER TQGSVALSVT QDPACKKPLG