Recombinant humaan HADH-eiwit

HADH, which belongs to the family of oxidoreductases, is important for converting certain fats to energy. This protein is an enzyme that catalyzes the chemical reaction. ((S) -3-hydroxyacyl-CoA + NAD+ <=>3-oxoacyl-CoA + NADH + H+) It is also involved in a process called fatty acid oxidation, in which several enzymes work in a step-wise fashion to break down (metabolize) fats and convert them to energy. Recombinant HADH protein was expressed in E. coli and purified by using conventional chromatography techniques.

Hydroxyacyl-CoA dehydrogenase, HADHSC, L-3-hydroxyacyl-Coenzyme A dehydrogenase, short chain, hydroxyacyl-Coenzyme A dehydrogenase, Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase, Short-chain 3-hydroxyacyl-CoA dehydrogenase, HADH1, SCHAD, HCDH, HAD, HAD1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

35.1 kDa (323aa) confirmed by MALDI-TOF

Hydroxyacyl-CoA dehydrogenase, HADHSC, L-3-hydroxyacyl-Coenzyme A dehydrogenase, short chain, hydroxyacyl-Coenzyme A dehydrogenase, Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase, Short-chain 3-hydroxyacyl-CoA dehydrogenase, HADH1, SCHAD, HCDH, HAD, HAD1, HADH 1, HAD 1, L 3 hydroxyacyl Coenzyme A dehydrogenase, Medium and short chain L 3 hydroxyacyl coenzyme A dehydrogenase, Hydroxyacyl CoA dehydrogenase, ATGP0501-10 µg, ATGP0501-20 µg, ATGP0501-50 µg, ATGP0501-100 µg, ATGP0501-250 µg, ATGP0501-500 µg, ATGP0501-1 mg, ATGP0501-010, ATGP0501-020, ATGP0501-050, ATGP0501-100, ATGP0501-250, ATGP0501-500, ATGP0501-01M

Tieu K, et al. (2004) Ann Neurol. 56 (1) :51-60; ; Vredendaal PJ, et al. (1996) Biochem Biophys Res Commun. 223 (3) :718-23.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl

Metabolism

AAH00306

Q16836

Human

MSSSSTASAS AKKIIVKHVT VIGGGLMGAG IAQVAAATGH TVVLVDQTED ILAKSKKGIE ESLRKVAKKK FAENPKAGDE FVEKTLSTIA TSTDAASVVH STDLVVEAIV ENLKVKNELF KRLDKFAAEH TIFASNTSSL QITSIANATT RQDRFAGLHF FNPVPVMKLV EVIKTPMTSQ KTFESLVDFS KALGKHPVSC KDTPGFIVNR LLVPYLMEAI RLYERGDASK EDIDTAMKLG AGYPMGPFEL LDYVGLDTTK FIVDGWHEMD AENPLHQPSP SLNKLVAENK FGKKTGEGFY KYK