Recombinant humaan HADHB-eiwit

HADHB is the beta subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the beta subunit catalyzing the 3-ketoacyl-CoA thiolase activity. This protein can also bind RNA and decreases the stability of some mRNAs. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation. Mutations in this gene result in trifunctional protein deficiency. Recombinant human HADHB protein, fused to His-tag at N-terminus, was expressed in E. coli.

Trifunctional enzyme subunit beta mitochondrial, Trifunctional enzyme subunit beta, mitochondrial, ECHB, MSTP029, MTPB, TP-BETA

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

49.9 kDa (464aa)

HADHB, Trifunctional enzyme subunit beta mitochondrial, Trifunctional enzyme subunit beta, mitochondrial, ECHB, MSTP029, MTPB, TP-BETA, ATGP2941-10 µg, ATGP2941-20 µg, ATGP2941-50 µg, ATGP2941-100 µg, ATGP2941-250 µg, ATGP2941-500 µg, ATGP2941-1 mg, ATGP2941-10, ATGP2941-20, ATGP2941-50, ATGP2941-100, ATGP2941-250, ATGP2941-500, ATGP2941-1

Purevsuren J., et al. (2009) Mol Genet Metab, PMID 19699128

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Metabolism

NP_000174

P55084

Human

AAPAVQT KTKKTLAKPN IRNVVVVDGV RTPFLLSGTS YKDLMPHDLA RAALTGLLHR TSVPKEVVDY IIFGTVIQEV KTSNVAREAA LGAGFSDKTP AHTVTMACIS ANQAMTTGVG LIASGQCDVI VAGGVELMSD VPIRHSRKMR KLMLDLNKAK SMGQRLSLIS KFRFNFLAPE LPAVSEFSTS ETMGHSADRL AAAFAVSRLE QDEYALRSHS LAKKAQDEGL LSDVVPFKVP GKDTVTKDNG IRPSSLEQMA KLKPAFIKPY GTVTAANSSF LTDGASAMLI MAEEKALAMG YKPKAYLRDF MYVSQDPKDQ LLLGPTYATP KVLEKAGLTM NDIDAFEFHE AFSGQILANF KAMDSDWFAE NYMGRKTKVG LPPLEKFNNW GGSLSLGHPF GATGCRLVMA AANRLRKEGG QYGLVAACAA GGQGHAMIVE AYPK