Recombinant humaan HARS-eiwit

HARS, also known as Histidyl-tRNA synthetase, function to catalyze the aminoacylation of tRNAs by their corresponding amino acids. HARS is from the class II family of aminoacyl-tRNA synthetases. It is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. HARS is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Recombinant human HARS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Histidyl-tRNA synthetase cytoplasmic, FLJ20491, HRS, HisRS, Histidine--tRNA ligase, Histidyl-tRNA synthetase, cytoplasmic

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by absorbance at 280 nm)

> 95% by SDS-PAGE

59.8 kDa (532aa) confirmed by MALDI-TOF

HARS, Histidyl-tRNA synthetase cytoplasmic, FLJ20491, HRS, HisRS, Histidine--tRNA ligase, Histidyl-tRNA synthetase, cytoplasmic, ATGP0726-10 µg, ATGP0726-20 µg, ATGP0726-50 µg, ATGP0726-100 µg, ATGP0726-250 µg, ATGP0726-500 µg, ATGP0726-1 mg, ATGP0726-10, ATGP0726-20, ATGP0726-50, ATGP0726-100, ATGP0726-250, ATGP0726-500, ATGP0726-1

Raben N., et al. (1992) Nucleic Acids Res. 20 (5) :1075-81. ; ; O'Hanlon TP., et al. (2002) Biochem Biophys Res Commun. 294 (3) :609-14.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.

Enzymes & Proteases

NP_002100

P12081

Human

MAERAAL EELVKLQGER VRGLKQQKAS AELIEEEVAK LLKLKAQLGP DESKQKFVLK TPKGTRDYSP RQMAVREKVF DVIIRCFKRH GAEVIDTPVF ELKETLMGKY GEDSKLIYDL KDQGGELLSL RYDLTVPFAR YLAMNKLTNI KRYHIAKVYR RDNPAMTRGR YREFYQCDFD IAGNFDPMIP DAECLKIMCE ILSSLQIGDF LVKVNDRRIL DGMFAICGVS DSKFRTICSS VDKLDKVSWE EVKNEMVGEK GLAPEVADRI GDYVQQHGGV SLVEQLLQDP KLSQNKQALE GLGDLKLLFE YLTLFGIDDK ISFDLSLARG LDYYTGVIYE AVLLQTPAQA GEEPLGVGSV AAGGRYDGLV GMFDPKGRKV PCVGLSIGVE RIFSIVEQRL EALEEKIRTT ETQVLVASAQ KKLLEERLKL VSELWDAGIK AELLYKKNPK LLNQLQYCEE AGIPLVAIIG EQELKDGVIK LRSVTSREEV DVRREDLVEE IKRRTGQPLC IC