Recombinant humaan harttroponine I3/TNNI3-eiwit

TNNI3, also known as Troponin I3, is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TNNI3 is responsible for inhibition of actomyosin ATPase activity. Therefore, TNNI3 is a key regulatory protein in cardiac muscle contraction and relaxation cycle. Recently, specific missense mutations of the TNNI3 gene have been shown to cause familial hypertrophic cardiomyopathy. Recombinant human TNNI3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography.

Troponin I3, CMD1FF, CMD2A, CMH7, cTnl, RCM1, TNNC1

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

26.4 kDa (233aa) confirmed by MALDI-TOF

Cardiac Troponin I, Troponin I3, CMD1FF, CMD2A, CMH7, cTnl, RCM1, TNNC1, ATGP3835-10 µg, ATGP3835-20 µg, ATGP3835-50 µg, ATGP3835-100 µg, ATGP3835-250 µg, ATGP3835-500 µg, ATGP3835-1 mg, ATGP3835-10, ATGP3835-20, ATGP3835-50, ATGP3835-100, ATGP3835-250, ATGP3835-500, ATGP3835-1

DW Sohn., et al. (2017) Korean Circ J. 47 (3) :413-417.; ; Elliot K., et al. (2000) J Biol Chem. 275 (29) :22069-74.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 30% glycerol, 0.1M NaCl, 0.1mM PMSF, 1mM DTT

Cardiovascular

NP_000354.4

P19429

Human

MADGSSD AAREPRPAPA PIRRRSSNYR AYATEPHAKK KSKISASRKL QLKTLLLQIA KQELEREAEE RRGEKGRALS TRCQPLELAG LGFAELQDLC RQLHARVDKV DEERYDIEAK VTKNITEIAD LTQKIFDLRG KFKRPTLRRV RISADAMMQA LLGARAKESL DLRAHLKQVK KEDTEKENRE VGDWRKNIDA LSGMEGRKKK FES