Recombinant humaan HAUS1-eiwit

HAuS1, also known as HAuS augmin-like complex subunit 1, is 1 of 8 subunits of the 390-kD human augmin complex, or HAuS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb augmentare, meaning to increase. The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly. Recombinant human HAuS1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

HAuS augmin-like complex subunit 1, CCDC5, HEI-C, HEIC, HsT1461, Coiled-coil domain containing 5, Enhancer of invasion-cluster

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

34.4 kDa (302aa) confirmed by MALDI-TOF

HAuS1, HAuS augmin-like complex subunit 1, CCDC5, HEI-C, HEIC, HsT1461, ATGP1491-10 µg, ATGP1491-20 µg, ATGP1491-50 µg, ATGP1491-100 µg, ATGP1491-250 µg, ATGP1491-500 µg, ATGP1491-1 mg, ATGP1491-10, ATGP1491-20, ATGP1491-50, ATGP1491-100, ATGP1491-250, ATGP1491-500, ATGP1491-1

Einarson M.B., et al. (2004) Mol. Cell. Biol. 24:3957-3971; ; Lawo S., et al. (2009) Curr. Biol. 19:816-826

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.2M NaCl, 2mM DTT

Cell Cycle

NP_612452

Q96CS2

Human

MEPQEE RETQVAAWLK KIFGDHPIPQ YEVNPRTTEI LHHLSERNRV RDRDVYLVIE DLKQKASEYE SEAKYLQDLL MESVNFSPAN LSSTGSRYLN ALVDSAVALE TKDTSLASFI PAVNDLTSDL FRTKSKSEEI KIELEKLEKN LTATLVLEKC LQEDVKKAEL HLSTERAKVD NRRQNMDFLK AKSEEFRFGI KAAEEQLSAR GMDASLSHQS LVALSEKLAR LKQQTIPLKK KLESYLDLMP NPSLAQVKIE EAKRELDSIE AELTRRVDMM EL