Recombinant humaan HAX-1-eiwit

HAX1 is known to associate with HS1, a substrate of Src family tyrosine kinases. It also interacts with the product of PKD2, mutations in which are associated with autosomal-dominant polycystic kidney disease, and with F-actin-binding protein, cortactin. It is associated with autosomal recessive severe congenital neutropenia, also known as Kostmann syndrome. Recombinant human HAX1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

HCLS1 associated protein X-1 isoform a, HCLSBP1, HS1BP1, SCN3

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

33.7 kDa (299aa) confirmed by MALDI-TOF

HAX1, HCLS1 associated protein X-1 isoform a, HCLSBP1, HS1BP1, SCN3, ATGP1969-10 µg, ATGP1969-20 µg, ATGP1969-50 µg, ATGP1969-100 µg, ATGP1969-250 µg, ATGP1969-500 µg, ATGP1969-1 mg, ATGP1969-10, ATGP1969-20, ATGP1969-50, ATGP1969-100, ATGP1969-250, ATGP1969-500, ATGP1969-1

Modem S. et al. (2008) J Cell Physiol. 214:14-19.; ; Klein C. et al. (2007) Nat Genet. 39:86-92.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl

Metabolism

NP_006109

O00165

Human

MGSSHHHHHH SSGLVPRGSH MSLFDLFRGF FGFPGPRSHR DPFFGGMTRD EDDDEEEEEE GGSWGRGNPR FHSPQHPPEE FGFGFSFSPG GGIRFHDNFG FDDLVRDFNS IFSDMGAWTL PSHPPELPGP ESETPGERLR EGQTLRDSML KYPDSHQPRI FGGVLESDAR SESPQPAPDW GSQRPFHRFD DVWPMDPHPR TREDNDLDSQ VSQEGLGPVL QPQPKSYFKS ISVTKITKPD GIVEERRTVV DSEGRTETTV TRHEADSSPR GDPESPRPPA LDDAFSILDL FLGRWFRSR