Recombinant humaan HBG1-eiwit

The gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1) . The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5' - epsilon - gamma-G - gamma-A - delta - beta - 3'. Recombinant human HBG1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Hemoglobin subunit gamma-1, HBGA, HBGR, HSGGL1, PRO2979

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

18 kDa (170aa) confirmed by MALDI-TOF

HBG1, Hemoglobin subunit gamma-1, HBGA, HBGR, HSGGL1, PRO2979, ATGP2153-10 µg, ATGP2153-20 µg, ATGP2153-50 µg, ATGP2153-100 µg, ATGP2153-250 µg, ATGP2153-500 µg, ATGP2153-1 mg, ATGP2153-10, ATGP2153-20, ATGP2153-50, ATGP2153-100, ATGP2153-250, ATGP2153-500, ATGP2153-1

Huisman TH, Kutlar F, et al. (1991) . Hemoglobin. 15 (5) :349-79.; ; Loudianos G, Moi P, et al. (1992) . Hemoglobin. 16 (6) :503-9.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol

Cardiovascular

NP_000550.2

P69891

Human

MGHFTEE DKATITSLWG KVNVEDAGGE TLGRLLVVYP WTQRFFDSFG NLSSASAIMG NPKVKAHGKK VLTSLGDATK HLDDLKGTFA QLSELHCDKL HVDPENFKLL GNVLVTVLAI HFGKEFTPEV QASWQKMVTA VASALSSRYH