Recombinant humaan HBG2-eiwit

Hemoglobin subunit gamma-2, also known as HBG2, belongs to the fetal hemoglobin subunit, which consists of two alpha chains together with two gamma chains. Increased fetal hemoglobin production in adults can ameliorate the clinical severity of sickle cell disease and beta-thalassemia major. Recombinant human HBG2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Hemoglobin subunit gamma-2, TNCY

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

18.5 kDa (170aa) confirmed by MALDI-TOF

HBG2, ATGP1927-10 µg, ATGP1927-20 µg, ATGP1927-50 µg, ATGP1927-100 µg, ATGP1927-250 µg, ATGP1927-500 µg, ATGP1927-1 mg, ATGP1927-10, ATGP1927-20, ATGP1927-50, ATGP1927-100, ATGP1927-250, ATGP1927-500, ATGP1927-1

Feng L., et al. (2004) Cell. 119:629-640.; ; Liebhaber S A., et al. (1981) Nature.290:26-29.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol,1mM DTT

Cardiovascular

NP_000175.1

P69892

Human

MGHFTEE DKATITSLWG KVNVEDAGGE TLGRLLVVYP WTQRFFDSFG NLSSASAIMG NPKVKAHGKK VLTSLGDAIK HLDDLKGTFA QLSELHCDKL HVDPENFKLL GNVLVTVLAI HFGKEFTPEV QASWQKMVTG VASALSSRYH