Recombinant humaan HBQ1-eiwit

Hemoglobin subunit theta-1, also known as HBQ1, belongs to the Hemoglobin family. Hemoglobin (Hgb) is a 66. 7 kDa protein coupled to four iron-binding, methenelinked tetrapyrrole rings (heme) . The globin portion of Hgb consists of two alpha chains and two beta chains arranged in pairs forming a tetramer. Each of the four globin chains covalently associates with a heme group. The bonds between alpha and beta chains are weaker than between similar globin chains, thereby forming a cleavage plane that is important for oxygen binding and release. High affinity for oxygen occurs upon relaxation of the alpha1-beta2 cleavage plane. Recombinant human HBQ1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Hemoglobin subunit theta-1, hemoglobin, theta 1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

17.9 kDa (165aa) confirmed by MALDI-TOF

HBQ1, Hemoglobin subunit theta-1, hemoglobin, theta 1, ATGP2230-10 µg, ATGP2230-20 µg, ATGP2230-50 µg, ATGP2230-100 µg, ATGP2230-250 µg, ATGP2230-500 µg, ATGP2230-1 mg, ATGP2230-10, ATGP2230-20, ATGP2230-50, ATGP2230-100, ATGP2230-250, ATGP2230-500, ATGP2230-1

Liebhaber S A., et al. (1981) Nature. 290:26-29; ; Sudha R., et al. (2004) J biol Chem. 279:20018-20027.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Cardiovascular

NP_005322

P09105

Human

MALSAED RALVRALWKK LGSNVGVYTT EALERTFLAF PATKTYFSHL DLSPGSSQVR AHGQKVADAL SLAVERLDDL PHALSALSHL HACQLRVDPA SFQLLGHCLL VTLARHYPGD FSPALQASLD KFLSHVISAL VSEYR