Recombinant humaan HDDC3-eiwit

HDDC3, also known as guanosine-3', 5'-bis (diphosphate) -pyrophosphohydrolase MESH1, contains an active site for ppGpp hydrolysis and a conserved His-Asp-box motif for Mn (2+) binding. Consistent with these structural data, HDDC3 efficiently catalyzes hydrolysis of guanosine 3', 5'-diphosphate (ppGpp) both in vitro and in vivo. HDDC3 also suppresses SpoT-deficient lethality and RelA-induced delayed cell growth in bacteria. Recombinant human HDDC3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Guanosine-3'5'-bis (diphosphate) -pyrophosphohydrolase MESH1, Guanosine-3',5'-bis (diphosphate) -pyrophosphohydrolase MESH1, MESH1, ppGpp

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

17.9 kDa (160aa) confirmed by MALDI-TOF

HDDC3, Guanosine-3'5'-bis (diphosphate) -pyrophosphohydrolase MESH1, Guanosine-3',5'-bis (diphosphate) -pyrophosphohydrolase MESH1, MESH1, ppGpp, ATGP1267-10 µg, ATGP1267-20 µg, ATGP1267-50 µg, ATGP1267-100 µg, ATGP1267-250 µg, ATGP1267-500 µg, ATGP1267-1 mg, ATGP1267-10, ATGP1267-20, ATGP1267-50, ATGP1267-100, ATGP1267-250, ATGP1267-500, ATGP1267-1

Sun D., et al. (2010) Nat. Struct. Mol. Biol. 17:1188-1194; ; Zody M.C., et al. (2006) Nature. 440:671-675

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT

Enzymes & Proteases

NP_940929

Q8N4P3

Human

MGSSHHHHHH SSGLVPRGSH MGSEAAQLLE AADFAARKHR QQRRKDPEGT PYINHPIGVA RILTHEAGIT DIVVLQAALL HDTVEDTDTT LDEVELHFGA QVRRLVEEVT DDKTLPKLER KRLQVEQAPH SSPGAKLVKL ADKLYNLRDL NRCTPEVKIQ