Recombinant humaan HDHD1A/PUDP-eiwit

HDHD1A, also known as haloacid dehalogenase-like hydrolase domain-containing protein 1A, belongs to the adiponutrin family. This protein is of interest because it is an X-linked gene that escapes X-inactivation. It is particularly important in the understanding of human X chromosome structural organization as well as the mechanism of X-inactivation. Recombinant human HDHD1A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Haloacid dehalogenase-like hydrolase domain-containing protein 1A, DXF68S1E, FAM16AX, GS1, pseudouridine 5'-phosphatase, HDHD1, Haloacid dehalogenase-like hydrolase domain-containing protein 1, Pseudouridine-5'-monophosphatase, 5'-PsiMPase

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

27.4 kDa (248aa) confirmed by MALDI-TOF

Pseudouridine-5'-monophosphatase, Pseudouridine 5'-phosphatase, Pseudouridine 5' phosphatase, Pseudouridine 5' monophosphatase, HDHD1, Haloacid dehalogenase-like hydrolase domain-containing protein 1A, Haloacid dehalogenase-like hydrolase domain-containing protein 1, Haloacid dehalogenase like hydrolase domain containing protein 1A, Haloacid dehalogenase like hydrolase domain containing protein 1, GS1, FAM16AX, DXF68S1E, 5'-PsiMPase, 5' PsiMPase, ATGP0797-10 µg, ATGP0797-20 µg, ATGP0797-50 µg, ATGP0797-100 µg, ATGP0797-250 µg, ATGP0797-500 µg, ATGP0797-1 mg, ATGP0797-010, ATGP0797-020, ATGP0797-050, ATGP0797-100, ATGP0797-250, ATGP0797-500, ATGP0797-01M

Salido E.C., et al. (1992), Am. J. Hum. Genet., 50: 303-316.; ; Soehnge H., et al. (1997) . Gene., 185: 257-263

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Enzymes & Proteases

NP_036212

Q08623

Human

MGSSHHHHHH SSGLVPRGSH MAAPPQPVTH LIFDMDGLLL DTERLYSVVF QEICNRYDKK YSWDVKSLVM GKKALEAAQI IIDVLQLPMS KEELVEESQT KLKEVFPTAA LMPGAEKLII HLRKHGIPFA LATSSGSASF DMKTSRHKEF FSLFSHIVLG DDPEVQHGKP DPDIFLACAK RFSPPPAMEK CLVFEDAPNG VEAALAAGMQ VVMVPDGNLS RDLTTKATLV LNSLQDFQPE LFGLPSYE