Recombinant humaan HDHD2-eiwit

Haloacid dehalogenase-like hydrolase domain containing 2, also known HDHD2, belongs to the HAD-like hydrolase superfamily. This family of hydrolase enzymes includes L-2-haloacid dehalogenase, epoxide hydrolases and phosphatases. HDHD2 has two active sites, an L-2-haloacid dehalogenase and a carboxylate group. The L-2-haloacid dehalogenase active site catalyzes the hydrolytic dehalogenation of D- and L-2-haloalkanoic acids, producing L- and D-2-hydroxyalkanoic acids. Recombinant human HDHD2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Haloacid dehalogenase-like hydrolase domain containing 2, 3110052N05Rik, DKFZp564D1378

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

30.6 kDa (279aa) confirmed by MALDI-TOF

HDHD2, Haloacid dehalogenase-like hydrolase domain containing 2, 3110052N05Rik, DKFZp564D1378, ATGP0818-10 µg, ATGP0818-20 µg, ATGP0818-50 µg, ATGP0818-100 µg, ATGP0818-250 µg, ATGP0818-500 µg, ATGP0818-1 mg, ATGP0818-10, ATGP0818-20, ATGP0818-50, ATGP0818-100, ATGP0818-250, ATGP0818-500, ATGP0818-1

Carstea E D., et al. (1993) Proc Matl Acad Sci uSA. 90:2002-2004.; ; Beiraqhi S., et al. (2007) Am J Hum Genet. 81:180-188.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Enzymes & Proteases

NP_115500

Q9H0R4

Human

MGSSHHHHHH SSGLVPRGSH MAACRALKAV LVDLSGTLHI EDAAVPGAQE ALKRLRGASV IIRFVTNTTK ESKQDLLERL RKLEFDISED EIFTSLTAAR SLLERKQVRP MLLVDDRALP DFKGIQTSDP NAVVMGLAPE HFHYQILNQA FRLLLDGAPL IAIHKARYYK RKDGLALGPG PFVTALEYAT DTKATVVGKP EKTFFLEALR GTGCEPEEAV MIGDDCRDDV GGAQDVGMLG ILVKTGKYRA SDEEKINPPP YLTCESFPHA VDHILQHLL