Recombinant humaan HDHD2B/LHPP-eiwit

Phospholysine phosphohistidine inorganic pyrophosphate phosphatase, also known as LHPP, is a member of the HAD-like hydrolase superfamily. It is a unique enzyme that hydrolyzes not only oxygen-phosphorus bonds in inorganic pyrophosphate but also nitrogen-phosphorus bonds in phospholysine, phosphohistidine and imidodiphosphate in vitro. LHPP is expressed in liver, kidney and moderately in brain. Recombinant human LHPP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Phospholysine phosphohistidine inorganic pyrophosphate phosphatase, HDHD2B

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

33.5 kDa (307aa) confirmed by MALDI-TOF

LHPP, phospholysine phosphohistidine inorganic pyrophosphate phosphatase, HDHD2B, ATGP1319-10 µg, ATGP1319-20 µg, ATGP1319-50 µg, ATGP1319-100 µg, ATGP1319-250 µg, ATGP1319-500 µg, ATGP1319-1 mg, ATGP1319-10, ATGP1319-20, ATGP1319-50, ATGP1319-100, ATGP1319-250, ATGP1319-500, ATGP1319-1

Neff C D., et al. (2009) Mol Psychiatry. 14:621-630.; ; Chen L., et al. (2005) Front Biosci. 10:1961-1976.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl, 0.1mM PMSF

Enzymes & Proteases

NP_071409.3

Q9H008

Human

MAP WGKRLAGVRG VLLDISGVLY DSGAGGGTAI AGSVEAVARL KRSRLKVRFC TNESQKSRAE LVGQLQRLGF DISEQEVTAP APAACQILKE QGLRPYLLIH DGVRSEFDQI DTSNPNCVVI ADAGESFSYQ NMNNAFQVLM ELEKPVLISL GKGRYYKETS GLMLDVGPYM KALEYACGIK AEVVGKPSPE FFKSALQAIG VEAHQAVMIG DDIVGDVGGA QRCGMRALQV RTGKFRPSDE HHPEVKADGY VDNLAEAVDL LLQHADK