Recombinant humaan HDHD4/NANP-eiwit

NANP (N-acylneuraminate-9-phosphatase), also known as HDHD4 (Haloacid dehalogenase-like hydrolase domain-containing protein 4), is belongs to the haloacid dehalogenase (HAD) family and is responsible for dephosphorylating N-acylneuraminate 9-phosphate to form N-acylneuraminate (N-acylneuraminate 9-phosphate + H2O = N-acylneuraminate + phosphate) . Characteristic of the HAD phosphatase family, the catalytic activity of NANP is dependent upon the presence of magnesium and is inhibited by vanadate and calcium. Recombinant human NANP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Neu5Ac-9-Pase, N-acylneuraminate-9-phosphatase, N-acetylneuraminic acid phosphatase, HDHD4, Haloacid dehalogenase-like hydrolase domain containing 4, C20orf147

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

31.9 kDa (284aa) confirmed by MALDI-TOF

NANP, N-acylneuraminate-9-phosphatase, HDHD4, C20orf147, Neu5Ac-9-Pase, N-acylneuraminate-9-phosphatase, ATGP0741-10 µg, ATGP0741-20 µg, ATGP0741-50 µg, ATGP0741-100 µg, ATGP0741-250 µg, ATGP0741-500 µg, ATGP0741-1 mg, ATGP0741-10, ATGP0741-20, ATGP0741-50, ATGP0741-100, ATGP0741-250, ATGP0741-500, ATGP0741-1

Hao J., et al. (2006) Biochem J. 397: 195-201.; ; Maliekal P., et al. (2006) Glycobiology. 16:165-172;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 100 mM NaCl

Metabolism

NP_689880

Q8TBE9

Human

MGLS RVRAVFFDLD NTLIDTAGAS RRGMLEVIKL LQSKYHYKEE AEIICDKVQV KLSKECFHPY NTCITDLRTS HWEEAIQETK GGAANRKLAE ECYFLWKSTR LQHMTLAEDV KAMLTELRKE VRLLLLTNGD RQTQREKIEA CACQSYFDAV VVGGEQREEK PAPSIFYYCC NLLGVQPGDC VMVGDTLETD IQGGLNAGLK ATVWINKNGI VPLKSSPVPH YMVSSVLELP ALLQSIDCKV SMST