Recombinant humaan hemoglobine zeta/HBZ-eiwit

Hemoglobin subunit zeta (HBZ) is belongs to the globin family. Zeta-globin is alpha-like hemoglobin. The HBZ polypeptide is synthesized in the yolk sac of the early embryo, while alpha-globin is produced throughout fetal and adult life. The HBZ gene includes five functional genes and two pseudogenes. The order of genes is: 5' - zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 -alpha-1 - theta1 - 3'. Recombinant human HBZ fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Zeta globin, Hemoglobin zeta chain, Hemoglobin zeta, Hemoglobin subunit zeta, HBZ-T1, HBZ2, HBZ1, HBZ 2, HBAZ

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

16.7 kDa (150aa) confirmed by MALDI-TOF

Zeta-globin, Zeta globin, Hemoglobin zeta chain, Hemoglobin zeta, Hemoglobin subunit zeta HBZ 2, Hemoglobin subunit zeta, HBZ-T1, HBZ2, HBZ1, HBZ 2, HBAZ, ATGP0528-10 µg, ATGP0528-20 µg, ATGP0528-50 µg, ATGP0528-100 µg, ATGP0528-250 µg, ATGP0528-500 µg, ATGP0528-1 mg, ATGP0528-010, ATGP0528-020, ATGP0528-050, ATGP0528-100, ATGP0528-250, ATGP0528-500, ATGP0528-01M

Lau ET., et al. (2001) Prenat. Diagn. 21 (7) : 529-39.; ; Kidd RD., et al. (2001) Biochemistry. 40 (51) :15669-75.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 100 mM NaCl, 20% glycerol

Immunology

NP_005323

P02008

Human