Recombinant humaan heparanase/HPSE-eiwit

Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. HPSE is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. In addition, this cleavage can release bioactive molecules from the extracellular matrix. Recombinant human HPSE protein, fused to His-tag at N-terminus, was expressed in E. coli.

Heparanase isoform 1 preproprotein, Heparanase, HPA, HPA1, HPR1, HPSE1, HSE1

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

60 kDa (531aa)

HPSE, Heparanase isoform 1 preproprotein, Heparanase, HPA, HPA1, HPR1, HPSE1, HSE1, ATGP2564-10 µg, ATGP2564-20 µg, ATGP2564-50 µg, ATGP2564-100 µg, ATGP2564-250 µg, ATGP2564-500 µg, ATGP2564-1 mg, ATGP2564-10, ATGP2564-20, ATGP2564-50, ATGP2564-100, ATGP2564-250, ATGP2564-500, ATGP2564-1

Okada Y., et al. (2002) J. Biol. Chem. 277:42488-42495.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea

Cancer

NP_006656

Q9Y251

Human

MGSSHHHHHH SSGLVPRGSH MGSQDVVDLD FFTQEPLHLV SPSFLSVTID ANLATDPRFL ILLGSPKLRT LARGLSPAYL RFGGTKTDFL IFDPKKESTF EERSYWQSQV NQDICKYGSI PPDVEEKLRL EWPYQEQLLL REHYQKKFKN STYSRSSVDV LYTFANCSGL DLIFGLNALL RTADLQWNSS NAQLLLDYCS SKGYNISWEL GNEPNSFLKK ADIFINGSQL GEDFIQLHKL LRKSTFKNAK LYGPDVGQPR RKTAKMLKSF LKAGGEVIDS VTWHHYYLNG RTATKEDFLN PDVLDIFISS VQKVFQVVES TRPGKKVWLG ETSSAYGGGA PLLSDTFAAG FMWLDKLGLS ARMGIEVVMR QVFFGAGNYH LVDENFDPLP DYWLSLLFKK LVGTKVLMAS VQGSKRRKLR VYLHCTNTDN PRYKEGDLTL YAINLHNVTK YLRLPYPFSN KQVDKYLLRP LGPHGLLSKS VQLNGLTLKM VDDQTLPPLM EKPLRPGSSL GLPAFSYSFF VIRNAKVAAC I