Recombinant humaan HERC5-eiwit

HERC5 is a member of the HERC family of ubiquitin ligases. It is a protein with a HECT domain and five RCC1 repeats. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. HERC5 gene lies in a cluster of HERC family genes on chromosome 4. It has been shown to exhibit antiviral activity towards HIV-1, influenza A virus and human papillomavirus. Recombinant human HERC5 protein, fused to His-tag at N-terminus, was expressed in E. coli.

E3 ISG15-protein ligase HERC5, CEB1, CEBP1

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

43 kDa (367aa)

HERC5, E3 ISG15-protein ligase HERC5, CEB1, CEBP1, ATGP2671-10 µg, ATGP2671-20 µg, ATGP2671-50 µg, ATGP2671-100 µg, ATGP2671-250 µg, ATGP2671-500 µg, ATGP2671-1 mg, ATGP2671-10, ATGP2671-20, ATGP2671-50, ATGP2671-100, ATGP2671-250, ATGP2671-500, ATGP2671-1

Woods MW, Kelly JN, et al. (2011) . Retrovirology. 8:95. ; ; Tang Y, Zhong G, et al. (2010) . J Immunol. 184 (10) :5777-90.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Immunology

NP_057407

Q9UII4

Human

FDLTVRR NHLIEDVLNQ LSQFENEDLR KELWVSFSGE IGYDLGGVKK EFFYCLFAEM IQPEYGMFMY PEGASCMWFP VKPKFEKKRY FFFGVLCGLS LFNCNVANLP FPLALFKKLL DQMPSLEDLK ELSPDLGKNL QTLLDDEGDN FEEVFYIHFN VHWDRNDTNL IPNGSSITVN QTNKRDYVSK YINYIFNDSV KAVYEEFRRG FYKMCDEDII KLFHPEELKD VIVGNTDYDW KTFEKNARYE PGYNSSHPTI VMFWKAFHKL TLEEKKKFLV FLTGTDRLQM KDLNNMKITF CCPESWNERD PIRALTCFSV LFLPKYSTME TVEEALQEAI NNNRGFG