Recombinant humaan HES2-eiwit

Hairy and enhancer of split 2, also known as HES2, is a member of the HES family and contains one basic helix-loop-helix (bHLH) domain and one orange domain. The HES family members form a complex with TLE, the mammalian homologue of Groucho, and this interaction is mediated by the carboxy terminal WRPW motif of the HES proteins. Activation of Notch signaling pathway leads to activation of HES family genes through the interaction between Notch intracellular domain and RBPSuH (CSL) . HES2 is expressed in a variety of adult and embryonic tissue. Recombinant human HES2 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Transcription factor HES-2, bHLHb40, hairy and enhancer of split 2

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

20.9 kDa (196aa)

HES2, Transcription factor HES-2, bHLHb40, hairy and enhancer of split 2, ATGP2260-10 µg, ATGP2260-20 µg, ATGP2260-50 µg, ATGP2260-100 µg, ATGP2260-250 µg, ATGP2260-500 µg, ATGP2260-1 mg, ATGP2260-10, ATGP2260-20, ATGP2260-50, ATGP2260-100, ATGP2260-250, ATGP2260-500, ATGP2260-1

Akazawa C., et al. (1992) J Biol Chem. 267:21879-21885.; ; Ishibashi M., et al. (1993) J Biochem. 215:645-652.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Epigenomics (Transcription & Translation)

NP_061962

Q9Y543

Human

MGSSHHHHHH SSGLVPRGSH MGSMGLPRRA GDAAELRKSL KPLLEKRRRA RINQSLSQLK GLILPLLGRE NSNCSKLEKA DVLEMTVRFL QELPASSWPT AAPLPCDSYR EGYSACVARL ARVLPACRVL EPAVSARLLE HLWRRAASAT LDGGRAGDSS GPSAPAPAPA SAPEPASAPV PSPPSPPCGP GLWRPW