Recombinant humaan Hexosaminidase A/HEXA-eiwit

HEXA is the alpha subunit of the lysosomal enzyme beta-hexosaminidase that, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Beta-hexosaminidase is composed of two subunits, alpha and beta, which are encoded by separate genes. Both beta-hexosaminidase alpha and beta subunits are members of family 20 of glycosyl hydrolases. Alpha subunit gene mutations lead to Tay-Sachs disease (GM2-gangliosidosis type I) . Recombinant human HEXA protein, fused to His-tag at N-terminus, was expressed in E. coli.

Beta-hexosaminidase subunit alpha, TSD, hexosaminidase A

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

52.9 kDa (464aa)

Hexosaminidase A, HEXA, beta-hexosaminidase subunit alpha, TSD, hexosaminidase A, ATGP1941-10 µg, ATGP1941-20 µg, ATGP1941-50 µg, ATGP1941-100 µg, ATGP1941-250 µg, ATGP1941-500 µg, ATGP1941-1 mg, ATGP1941-10, ATGP1941-20, ATGP1941-50, ATGP1941-100, ATGP1941-250, ATGP1941-500, ATGP1941-1

Nakano T., et al. (1988) J. Neurochem. 51:984-987

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M uREA, 10% glycerol

Neuroscience

NP_000511.1

P06865

Human

TLEKNVL VVSVVTPGCN QLPTLESVEN YTLTINDDQC LLLSETVWGA LRGLETFSQL VWKSAEGTFF INKTEIEDFP RFPHRGLLLD TSRHYLPLSS ILDTLDVMAY NKLNVFHWHL VDDPSFPYES FTFPELMRKG SYNPVTHIYT AQDVKEVIEY ARLRGIRVLA EFDTPGHTLS WGPGIPGLLT PCYSGSEPSG TFGPVNPSLN NTYEFMSTFF LEVSSVFPDF YLHLGGDEVD FTCWKSNPEI QDFMRKKGFG EDFKQLESFY IQTLLDIVSS YGKGYVVWQE VFDNKVKIQP DTIIQVWRED IPVNYMKELE LVTKAGFRAL LSAPWYLNRI SYGPDWKDFY VVEPLAFEGT PEQKALVIGG EACMWGEYVD NTNLVPRLWP RAGAVAERLW SNKLTSDLTF AYERLSHFRC ELLRRGVQAQ PLNVGFCEQE FEQT