Recombinant humaan HFE-eiwit

HFE, also known as HLA-H, belongs to the MHC class I family. This protein contains 1 Ig-like C1-type (immunoglobulin-like) domain. HFE is a membrane protein that is similar to MHC class I-type proteins and associates with beta-2 microglobulin (beta2M) . It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor (TFR) with transferrin. It binds to transferrin receptor (TFR) and reduces its affinity for iron-loaded transferrin. Recombinant human HFE protein, fused to His-tag at N-terminus, was expressed in E. coli.

Hereditary hemochromatosis protein, HFE1, HH, HLA-H, MVCD7, TFQTL2

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

35.7 kDa (308aa)

HFE, Hereditary hemochromatosis protein, HFE1, HH, HLA-H, MVCD7, TFQTL2, ATGP1869-10 µg, ATGP1869-20 µg, ATGP1869-50 µg, ATGP1869-100 µg, ATGP1869-250 µg, ATGP1869-500 µg, ATGP1869-1 mg, ATGP1869-10, ATGP1869-20, ATGP1869-50, ATGP1869-100, ATGP1869-250, ATGP1869-500, ATGP1869-1

Feder J.N., et al. (1998) Proc. Natl. Acad. Sci. u.S.A. 95:1472-1477; ; West, A P, et al. (2000) J. Biol. Chem. (uNITED STATES) 275 (49) : 38135-8.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Metabolism

NP_000401.1

Q30201

Human

RLLRSH SLHYLFMGAS EQDLGLSLFE ALGYVDDQLF VFYDHESRRV EPRTPWVSSR ISSQMWLQLS QSLKGWDHMF TVDFWTIMEN HNHSKESHTL QVILGCEMQE DNSTEGYWKY GYDGQDHLEF CPDTLDWRAA EPRAWPTKLE WERHKIRARQ NRAYLERDCP AQLQQLLELG RGVLDQQVPP LVKVTHHVTS SVTTLRCRAL NYYPQNITMK WLKDKQPMDA KEFEPKDVLP NGDGTYQGWI TLAVPPGEEQ RYTCQVEHPG LDQPLIVIWE PSPSGTLV