Recombinant humaan HIBCH-eiwit

HIBCH is the enzyme responsible for hydrolysis of both HIBYL-CoA and beta-hydroxypropionyl-CoA. Mutations in this gene have been associated with 3-hyroxyisobutyryl-CoA hydrolase deficiency. Alternative splicing results in multiple transcript variants. Recombinant human HIBCH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

3-hydroxyisobutyryl-CoA hydrolase mitochondrial, 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial, HIBYLCOAH

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

42.1 kDa (379aa) confirmed by MALDI-TOF

HIBCH, 3-hydroxyisobutyryl-CoA hydrolase mitochondrial, 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial, HIBYLCOAH, ATGP1469-10 µg, ATGP1469-20 µg, ATGP1469-50 µg, ATGP1469-100 µg, ATGP1469-250 µg, ATGP1469-500 µg, ATGP1469-1 mg, ATGP1469-10, ATGP1469-20, ATGP1469-50, ATGP1469-100, ATGP1469-250, ATGP1469-500, ATGP1469-1

Hendrickson, S.L. et al. (2010) PLoS ONE 5 (9), E12862; ; Wu, C. et al. (2007) Proteomics 7 (11), 1775-1785

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.2M NaCl, 1mM DTT

Signal Transduction

NP_055177

Q6NVY1

Human

MGSSHHHHHH SSGLVPRGSH MGSHMDAAEE VLLEKKGCTG VITLNRPKFL NALTLNMIRQ IYPQLKKWEQ DPETFLIIIK GAGGKAFCAG GDIRVISEAE KAKQKIAPVF FREEYMLNNA VGSCQKPYVA LIHGITMGGG VGLSVHGQFR VATEKCLFAM PETAIGLFPD VGGGYFLPRL QGKLGYFLAL TGFRLKGRDV YRAGIATHFV DSEKLAMLEE DLLALKSPSK ENIASVLENY HTESKIDRDK SFILEEHMDK INSCFSANTV EEIIENLQQD GSSFALEQLK VINKMSPTSL KITLRQLMEG SSKTLQEVLT MEYRLSQACM RGHDFHEGVR AVLIDKDQSP KWKPADLKEV TEEDLNNHFK SLGSSDLKF