Recombinant humaan HIF-1 alfa/HIF1A-eiwit

Hypoxia-inducible factor-1 (HIF-1), identified as one of the transcription factors, has been found to play an essential role in oxygen homeostasis. HIF-1 is a heterodimer composed of HIF-1 beta subunit and one of three subunits (Hif-1 alpha, Hif-2 alpha or Hif-3 alpha) . The activation of Hif-1 alpha is closely associated with a variety of tumors and oncogenic pathways. Hif-1 alpha consists of DNA binding domain (DBD domain), dimerization domain and C-terminal regulatory domains, including two transactivation domains (TAD), an oxygen-dependent degradation (ODD) domain, and inhibitory domains. Recombinant human Hif-1 alpha (1-85) protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Hypoxia inducible factor 1 subunit alpha, Hypoxia-inducible factor 1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, bHLHe78, Member of PAS protein 1, PAS domain-containing protein 8, PASD8, MOP1

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

11.8 kDa (105aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

HIF1 a, Hypoxia-inducible factor 1-alpha, BHLHE78, MOP1, PASD8, Hypoxia-inducible factor 1-alpha, ATGP0730-10 µg, ATGP0730-20 µg, ATGP0730-50 µg, ATGP0730-100 µg, ATGP0730-250 µg, ATGP0730-500 µg, ATGP0730-1 mg, ATGP0730-10, ATGP0730-20, ATGP0730-50, ATGP0730-100, ATGP0730-250, ATGP0730-500, ATGP0730-1

Okuyama H., et al. (2006) J Biol Chem. 281 (22) :15554-63.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 0.2M NaCl, 1mM EDTA

Epigenomics (Transcription & Translation)

NP_001521.1

Q16665

Human

MEGAGGANDK KKISSERRKE KSRDAARSRR SKESEVFYEL AHQLPLPHNV SSHLDKASVM RLTISYLRVR KLLDAGDLDI EDDMK