Recombinant humaan Histone Deacetylase 8/HDAC8-eiwit

HDAC8, also known as histone deacetylase 8, belongs to class 1 of the histone deacetylase/acuc/apha family. This protein is biologically involved in skull morphogenesis and metabolic control of the ERR-alpha/PGC1-alpha transcriptional complex. Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. Recombinant human HDAC8 protein, fused to His-tag at C-terminus, was expressed in Sf9 insect cell and purified by using conventional chromatography.

Protein deacetylase HDAC8, Protein decrotonylase HDAC8, Wilson-Turner X-linked mental retardation syndrome HDACL1, CDA07, WTS, MRXS6, RPD3, KDAC8

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

<1 EU per 1ug of protein (determined by LAL method)

> 80% by SDS-PAGE

42.6 kDa (383aa)

Histone Deacetylase8, Histone deacetylase 8, CDA07, HD8, HDACL1, RPD3, ATGP1329-10 µg, ATGP1329-20 µg, ATGP1329-50 µg, ATGP1329-100 µg, ATGP1329-250 µg, ATGP1329-500 µg, ATGP1329-1 mg, ATGP1329-10, ATGP1329-20, ATGP1329-50, ATGP1329-100, ATGP1329-250, ATGP1329-500, ATGP1329-1

Hu E., et al. (2000) J. Biol. Chem. 275:15254-15264; ; Buggy J.J., et al. (2000) Biochem. J. 350:199-205

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Epigenomics (Transcription & Translation)

NP_060956

Q9BY41

Human