Recombinant humaan Histone H3,3/H3-3A-eiwit

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. H3F3A is a replication-independent member of the histone H3 family. Recombinant human H3F3A protein, fused to His-tag at N-terminus, was expressed in E. coli.

Histone H3.3, H3.3A, H3F3

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

17.7 kDa (159aa)

H3F3A, Histone H3.3, H3.3A, H3F3, ATGP2083-10 µg, ATGP2083-20 µg, ATGP2083-50 µg, ATGP2083-100 µg, ATGP2083-250 µg, ATGP2083-500 µg, ATGP2083-1 mg, ATGP2083-10, ATGP2083-20, ATGP2083-50, ATGP2083-100, ATGP2083-250, ATGP2083-500, ATGP2083-1

Tagami H., et al. (2004) Cell. 116:51-61; ; Daury L N., et al. (2006) EMBO Rep. 7:66-71

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Epigenomics (Transcription & Translation)

NP_002098

P84243

Human

MGSSHHHHHH SSGLVPRGSH MGSMARTKQT ARKSTGGKAP RKQLATKAAR KSAPSTGGVK KPHRYRPGTV ALREIRRYQK STELLIRKLP FQRLVREIAQ DFKTDLRFQS AAIGALQEAS EAYLVGLFED TNLCAIHAKR VTIMPKDIQL ARRIRGERA