Recombinant humaan HLA-C eiwit

HLA-C belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin) . The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from endoplasmic reticulum lumen. They are expressed in nearly all cells. Recombinant human HLA-C protien, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

HLA class I histocompatibility antigen Cw-1 alpha chain, HLA class I histocompatibility antigen, Cw-1 alpha chain, D6S204, HLA-JY3, HLC-C, PSORS1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

34.9 kDa (307aa) confirmed by MALDI-TOF

HLA-C, ATGP2222-10 µg, ATGP2222-20 µg, ATGP2222-50 µg, ATGP2222-100 µg, ATGP2222-250 µg, ATGP2222-500 µg, ATGP2222-1 mg, ATGP2222-10, ATGP2222-20, ATGP2222-50, ATGP2222-100, ATGP2222-250, ATGP2222-500, ATGP2222-1

Xu Y., et al. (2009) Tissue Antigens. 74:453-455; ; Zou H.Y., et al. (2009) Tissue Antigens. 74:455-456

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Immunology

NP_002108

P10321

Human

CSHSMRY FDTAVSRPGR GEPRFISVGY VDDTQFVRFD SDAASPRGEP RAPWVEQEGP EYWDRETQKY KRQAQADRVS LRNLRGYYNQ SEDGSHTLQR MSGCDLGPDG RLLRGYDQSA YDGKDYIALN EDLRSWTAAD TAAQITQRKL EAARAAEQLR AYLEGTCVEW LRRYLENGKE TLQRAEPPKT HVTHHPLSDH EATLRCWALG FYPAEITLTW QRDGEDQTQD TELVETRPAG DGTFQKWAAV VVPSGQEQRY TCHMQHEGLQ EPLTLSWEPS SQPTIPI