Recombinant humaan HLA-DOB-eiwit

HLA-DOB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages) . The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Recombinant human HLA-DOB protein, fused to His-tag at N-terminus, was expressed in E. coli.

Major histocompatibility complex class II DO beta, Major histocompatibility complex, class II, DO beta, DOB

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

25.2 kDa (222aa)

HLA-DOB, Major histocompatibility complex class II DO beta, Major histocompatibility complex, class II, DO beta, DOB, ATGP2683-10 µg, ATGP2683-20 µg, ATGP2683-50 µg, ATGP2683-100 µg, ATGP2683-250 µg, ATGP2683-500 µg, ATGP2683-1 mg, ATGP2683-10, ATGP2683-20, ATGP2683-50, ATGP2683-100, ATGP2683-250, ATGP2683-500, ATGP2683-1

Naruse T.K., et al. (2002) Tissue Antigens. 59:512-519; ; Beck S., et al. (1996) J. Mol. Biol. 255:1-13;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Immunology

NP_002111

P13765

Human

TDSPED FVIQAKADCY FTNGTEKVQF VVRFIFNLEE YVRFDSDVGM FVALTKLGQP DAEQWNSRLD LLERSRQAVD GVCRHNYRLG APFTVGRKVQ PEVTVYPERT PLLHQHNLLH CSVTGFYPGD IKIKWFLNGQ EERAGVMSTG PIRNGDWTFQ TVVMLEMTPE LGHVYTCLVD HSSLLSPVSV EWRAQSEYSW RK