Recombinant humaan HLA-DRA eiwit

Major histocompatibility complex, class II, DR alpha, also known as HLA-DRA, binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Recombinant human HLA-DRA, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Major histocompatibility complex class II DR alpha, Major histocompatibility complex, class II, DR alpha, HLA-DA

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

24.3 kDa (212aa) confirmed by MALDI-TOF

HLA-DR, Major histocompatibility complex class II DR alpha, Major histocompatibility complex, class II, DR alpha, HLA-DA, ATGP2587-10 µg, ATGP2587-20 µg, ATGP2587-50 µg, ATGP2587-100 µg, ATGP2587-250 µg, ATGP2587-500 µg, ATGP2587-1 mg, ATGP2587-10, ATGP2587-20, ATGP2587-50, ATGP2587-100, ATGP2587-250, ATGP2587-500, ATGP2587-1

De Gassart A., et al. (2008) Proc. Natl. Acad. Sci. u.S.A. 105:3491-3496; ; Lapaque N., et al. (2009) J. Biol. Chem. 284:7007-7016;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Immunology

NP_061984.2

P01903

Human

IKEEHVIIQ AEFYLNPDQS GEFMFDFDGD EIFHVDMAKK ETVWRLEEFG RFASFEAQGA LANIAVDKAN LEIMTKRSNY TPITNVPPEV TVLTNSPVEL REPNVLICFI DKFTPPVVNV TWLRNGKPVT TGVSETVFLP REDHLFRKFH YLPFLPSTED VYDCRVEHWG LDEPLLKHWE FDAPSPLPET TE