Recombinant humaan HLA-DRB1-eiwit

HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages) . The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail. Recombinant human HLA-DRB1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Major histocompatibility complex class II DR beta 1, Major histocompatibility complex, class II, DR beta 1, DRB1, HLA DRB1, HLA-DR1B, HLA-DRB1

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

25.2 kDa (219aa)

HLA-DRB1, Major histocompatibility complex class II DR beta 1, Major histocompatibility complex, class II, DR beta 1, DRB1, HLA DRB1, HLA-DR1B, HLA-DRB1, ATGP2585-10 µg, ATGP2585-20 µg, ATGP2585-50 µg, ATGP2585-100 µg, ATGP2585-250 µg, ATGP2585-500 µg, ATGP2585-1 mg, ATGP2585-10, ATGP2585-20, ATGP2585-50, ATGP2585-100, ATGP2585-250, ATGP2585-500, ATGP2585-1

Menendez-Benito V., et al. (2007) Immunity. 26:1-3; ; De Gassart A., et al. (2008) Proc. Natl. Acad. Sci. u.S.A. 105:3491-3496;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Immunology

NP_002115

P01911

Human

MGSSHHHHHH SSGLVPRGSH MGDTRPRFLW QPKRECHFFN GTERVRFLDR YFYNQEESVR FDSDVGEFRA VTELGRPDAE YWNSQKDILE QARAAVDTYC RHNYGVVESF TVQRRVQPKV TVYPSKTQPL QHHNLLVCSV SGFYPGSIEV RWFLNGQEEK AGMVSTGLIQ NGDWTFQTLV MLETVPRSGE VYTCQVEHPS VTSPLTVEWR ARSESAQSK