Recombinant humaan HLA-F eiwit

HLA class I histocompatibility antigen, alpha chain F isoform 1 precursor, also known as HLA-F, is member of the MHC family that are specifically involved in the presentation of antigens to the T cell receptor. There are two classes of HLA antigens. HLA-F, belong to the Class I molecules. Class I molecules are expressed in nearly all cells and play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum. Recombinant human HLA-F, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

HLA class I histocompatibility antigen alpha chain F isoform 1, HLA class I histocompatibility antigen, alpha chain F isoform 1, CDA12, HLA-5.4, HLA-CDA12, HLAF

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

35.1 kDa (307aa) confirmed by MALDI-TOF

HLA-F, HLA class I histocompatibility antigen alpha chain F isoform 1, HLA class I histocompatibility antigen, alpha chain F isoform 1, CDA12, HLA-5.4, HLA-CDA12, HLAF, ATGP2732-10 µg, ATGP2732-20 µg, ATGP2732-50 µg, ATGP2732-100 µg, ATGP2732-250 µg, ATGP2732-500 µg, ATGP2732-1 mg, ATGP2732-10, ATGP2732-20, ATGP2732-50, ATGP2732-100, ATGP2732-250, ATGP2732-500, ATGP2732-1

Kropshofer H., et al. (1998) EMBO J. 17: 2971-2981.; ; Brunet A., et al. (2000) J Biol Chem. 275: 37062-37071.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Immunology

NP_001091949.1

P30511

Human

GSHSLRY FSTAVSRPGR GEPRYIAVEY VDDTQFLRFD SDAAIPRMEP REPWVEQEGP QYWEWTTGYA KANAQTDRVA LRNLLRRYNQ SEAGSHTLQG MNGCDMGPDG RLLRGYHQHA YDGKDYISLN EDLRSWTAAD TVAQITQRFY EAEEYAEEFR TYLEGECLEL LRRYLENGKE TLQRADPPKA HVAHHPISDH EATLRCWALG FYPAEITLTW QRDGEEQTQD TELVETRPAG DGTFQKWAAV VVPPGEEQRY TCHVQHEGLP QPLILRWEQS PQPTIPI