Recombinant humaan HLA-G eiwit

HLA-G, also known as HLA class I histocompatibility antigen, alpha chain G, belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin) . The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. HLA-G is a non-classical class-I HLA molecule associated with anti-inflammatory and immuno-modulatory properties which interacts with inhibitory receptors (ILT2/ILT4/KIR2DL4) present on various immune cells. It inhibits proliferation of B cells, T cells and natural killer cells; and also induces regulatory T cells. Recombinant human HLA-G, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

HLA class I histocompatibility antigen, alpha chain G, MHC-G

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

> 90% by SDS-PAGE

35.3 kDa (309aa) confirmed by MALDI-TOF

HLA-G, ATGP3743-10 µg, ATGP3743-20 µg, ATGP3743-50 µg, ATGP3743-100 µg, ATGP3743-250 µg, ATGP3743-500 µg, ATGP3743-1 mg, ATGP3743-10, ATGP3743-20, ATGP3743-50, ATGP3743-100, ATGP3743-250, ATGP3743-500, ATGP3743-1

Poomarimuthu M., et al. (2017) Pediatr Rheumatol Online J. 15 (1) :10.; ; Sanders SK., et al. (1991) J Exp Med. 174 (3) :737-40.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Immunology

NP_002118.1

P17693

Human

GSHSM RYFSAAVSRP GRGEPRFIAM GYVDDTQFVR FDSDSACPRM EPRAPWVEQE GPEYWEEETR NTKAHAQTDR MNLQTLRGYY NQSEASSHTL QWMIGCDLGS DGRLLRGYEQ YAYDGKDYLA LNEDLRSWTA ADTAAQISKR KCEAANVAEQ RRAYLEGTCV EWLHRYLENG KEMLQRADPP KTHVTHHPVF DYEATLRCWA LGFYPAEIIL TWQRDGEDQT QDVELVETRP AGDGTFQKWA AVVVPSGEEQ RYTCHVQHEG LPEPLMLRWK QSSLPTIPI