Recombinant humaan HMGA1-eiwit

High mobility group protein HMG-I/HMG-Y, also known as HMGA1, is a member of the non-histone chromosomal high mobility group protein (HMG) family. HMGA1 consists of a highly conserved AT-hook DNA-binding domain that mediates binding to AT-rich sequences in the minor groove of chromosomal DNA. It functions as architectural chromatin-binding transcription factor altering the conformation of DNA by modulating nuclear protein-DNA complexes. It is involved in many cellular processes including growth regulation, viral induction of beta-IFN gene and regulation of inducible gene transcription. Recombinant human HMGA1 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

High mobility group protein HMG-I/HMG-Y, HMG-R, HMGA1A, HMGIY

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by BCA assay)

> 90% by SDS-PAGE

12.7 kDa (115aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

HMGA1, High mobility group protein HMG-I/HMG-Y, HMG-R, HMGA1A, HMGIY, ATGP1140-10 µg, ATGP1140-20 µg, ATGP1140-50 µg, ATGP1140-100 µg, ATGP1140-250 µg, ATGP1140-500 µg, ATGP1140-1 mg, ATGP1140-10, ATGP1140-20, ATGP1140-50, ATGP1140-100, ATGP1140-250, ATGP1140-500, ATGP1140-1

Chiappetta G., et al. (2004) Clin Cancer Res. 10 (22) :7637-44.; ; Massaad Massade L., et al. (2002) Biochemistry. 41:2760-2768.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.2M NaCl

Signal Transduction

NP_665906.1

P17096

Human