Recombinant humaan HMGB3-eiwit

HMGB3 belongs to the high mobility group (HMG) protein superfamily. Like HMG1 (MIM 163905) and HMG2 (MIM 163906), HMGB3 contains DNA-binding HMG box domains and is classified into the HMG box subfamily. Members of the HMG box subfamily are thought to play a fundamental role in DNA replication, nucleosome assembly and transcription. Recombinant human HMGB3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

High mobility group protein B3, HMG-2a, HMG-4, HMG2A, HMG4

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

22.8 kDa (203aa) confirmed by MALDI-TOF

HMGB3, High mobility group protein B3, HMG-2a, HMG-4, HMG2A, HMG4, ATGP2238-10 µg, ATGP2238-20 µg, ATGP2238-50 µg, ATGP2238-100 µg, ATGP2238-250 µg, ATGP2238-500 µg, ATGP2238-1 mg, ATGP2238-10, ATGP2238-20, ATGP2238-50, ATGP2238-100, ATGP2238-250, ATGP2238-500, ATGP2238-1

Nemeth, M.J., et al. (2006) Proc. Natl. Acad. Sci. u.S.A. 103 (37), 13783-13788; ; Ross, M.T., et al. (2005) Nature 434 (7031), 325-337

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_005333

O15347

Human

MAKGDPK KPKGKMSAYA FFVQTCREEH KKKNPEVPVN FAEFSKKCSE RWKTMSGKEK SKFDEMAKAD KVRYDREMKD YGPAKGGKKK KDPNAPKRPP SGFFLFCSEF RPKIKSTNPG ISIGDVAKKL GEMWNNLNDS EKQPYITKAA KLKEKYEKDV ADYKSKGKFD GAKGPAKVAR KKV