Recombinant humaan HMGCL-eiwit

HMGCL, also known as hydroxymethylglutaryl-CoA lyase, mitochondrial isoform 1, is a mitochondrial matrix protein that belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes the final step of leucine degradation and plays a key role in ketone body formation. Multiple isoforms of the proteins are known due to alternative splicing. The major isoform (isoform 1) is most highly expressed in the liver whereas isoform 2 is found in energy-demanding tissues including the brain, heart, and skeletal muscle. Recombinant human HMGCL protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Hydroxymethylglutaryl-CoA lyase, mitochondrial isoform 1, HMGCL, HL

Baculovirus

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

32.5 kDa (305aa)

HMGCL, Hydroxymethylglutaryl-CoA lyase, mitochondrial isoform 1, HMGCL, HL, ATGP3658-10 µg, ATGP3658-20 µg, ATGP3658-50 µg, ATGP3658-100 µg, ATGP3658-250 µg, ATGP3658-500 µg, ATGP3658-1 mg, ATGP3658-10, ATGP3658-20, ATGP3658-50, ATGP3658-100, ATGP3658-250, ATGP3658-500, ATGP3658-1

Fu Z., et al, (2010) J Biol Chem. 285:26341-26349.; ; Montgomery C., et al, (2011) Arch Biochem Biophys. 511:48-55.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT

Signal Transduction

NP_000182

P35914

Human

Met