Recombinant humaan HMGN3-eiwit

HMGN3, also known as high mobility group nucleosomal binding domain 3, binds thyroid hormone receptor beta, but only in the presence of thyroid hormone. Thyroid hormone receptors are hormone-dependent transcription factors that regulate expression of a variety of specific target genes. It is thought to reduce the compactness of the chromatin fiber in nucleosomes, thereby enhancing transcription from chromatin templates. Recombinant human HMGN3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

High mobility group nucleosomal binding domain 3, PNAS-24, PNAS-25, TRIP7

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by BCA assay)

> 95% by SDS-PAGE

10.8 kDa (100aa) confirmed by MALDI-TOF

HMGN3, High mobility group nucleosomal binding domain 3, PNAS-24, PNAS-25, TRIP7, ATGP2796-10 µg, ATGP2796-20 µg, ATGP2796-50 µg, ATGP2796-100 µg, ATGP2796-250 µg, ATGP2796-500 µg, ATGP2796-1 mg, ATGP2796-10, ATGP2796-20, ATGP2796-50, ATGP2796-100, ATGP2796-250, ATGP2796-500, ATGP2796-1

Lee JW. et al. (1995) Mol Endocrinol. 9:243-254; ; West KL. et al. (2001) J Biol Chem. 276:25959-25969.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH7.0) containing 10% glycerol

Epigenomics (Transcription & Translation)

NP_620058

Q15651

Human

MGSSHHHHHH SSGLVPRGSH MGSMPKRKSP ENTEGKDGSK VTKQEPTRRS ARLSAKPAPP KPEPKPRKTS AKKEPGAKIS RGAKGKKEEK QEAGKEGTEN