Recombinant humaan HNRNPC-eiwit

HNRNPC, also known as hnRNP C1/C2, belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs) . These proteins are associated with pre mRNAs in the nucleus and appear to influence pre mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. Recombinant human HNRNPC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRNP, HNRPC, SNRPC

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

34.5 kDa (313aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

HNRNPC, Heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRNP, HNRPC, SNRPC, ATGP0965-10 µg, ATGP0965-20 µg, ATGP0965-50 µg, ATGP0965-100 µg, ATGP0965-250 µg, ATGP0965-500 µg, ATGP0965-1 mg, ATGP0965-10, ATGP0965-20, ATGP0965-50, ATGP0965-100, ATGP0965-250, ATGP0965-500, ATGP0965-1

Hayakawa H., et al. (2010) Biochem Biophys Res Commun. 403 (2) :220-4.; ; Stone JR., et al. (2002) J Biol Chem. 277 (18) :15621-8.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 30% glycerol

Signal Transduction

NP_004491

P07910

Human

MASNVTNKTD PRSMNSRVFI GNLNTLVVKK SDVEAIFSKY GKIVGCSVHK GFAFVQYVNE RNARAAVAGE DGRMIAGQVL DINLAAEPKV NRGKAGVKRS AAEMYGSSFD LDYDFQRDYY DRMYSYPARV PPPPPIARAV VPSKRQRVSG NTSRRGKSGF NSKSGQRGSS KSGKLKGDDL QAIKKELTQI KQKVDSLLEN LEKIEKEQSK QAVEMKNDKS EEEQSSSSVK KDETNVKMES EGGADDSAEE GDLLDDDDNE DRGDDQLELI KDDEKEAEEG EDDRDSANGE DDS