Recombinant humaan HOPX-eiwit

HOPX, also known as homeodomain-only protein, acts via its interaction with SRF, thereby modulating the expression of SRF-dependent cardiac-specific genes and cardiac development. This protein prevents SRF-dependent transcription either by inhibiting SRF binding to DNA or by recruiting histone deacetylase (HDAC) proteins that prevent transcription by SRF. Overexpression causes cardiac hypertrophy. Recombinant human HOPX protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Homeodomain-only protein, CAMEO, HOD, HOP, LAGY, NECC1, OB1, SMAP31, TOTO

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

10.8 kDa (97aa) confirmed by MALDI-TOF

HOPX, Homeodomain-only protein, CAMEO, HOD, HOP, LAGY, NECC1, OB1, SMAP31, TOTO, ATGP1639-10 µg, ATGP1639-20 µg, ATGP1639-50 µg, ATGP1639-100 µg, ATGP1639-250 µg, ATGP1639-500 µg, ATGP1639-1 mg, ATGP1639-10, ATGP1639-20, ATGP1639-50, ATGP1639-100, ATGP1639-250, ATGP1639-500, ATGP1639-1

Adu J., et al. (2002) Mech. Dev. 119:S43-S47; ; Lemaire F., et al. (2007) Br. J. Cancer. 91:258-261

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Epigenomics (Transcription & Translation)

NP_631957

Q9BPY8

Human

MGSSHHHHHH SSGLVPRGSH MGSHMSAETA SGPTEDQVEI LEYNFNKVDK HPDSTTLCLI AAEAGLSEEE TQKWFKQRLA KWRRSEGLPS ECRSVTD