Recombinant humaan HPCAL1-eiwit

HPCAL1, also known as VILIP-3, is a member of neuron-specific calcium-binding proteins family found in the retina and brain. It is highly similar to human hippocalcin protein and nearly identical to the rat and mouse hippocalcin like-1 proteins. It is involved in the calcium-dependent regulation of rhodopsin phosphorylation and may be of relevance for neuronal signalling in the central nervous system. Recombinant human HPCAL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Hippocalcin-like 1, BDR1, Calcium-binding protein BDR-1, Hippocalcin-like protein 1, HLP2, VILIP-3, visinin-like protein 3, VILIP3

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

24.4 kDa (213aa) confirmed by MALDI-TOF

HPCAL1, Hippocalcin-like 1, BDR1, Calcium-binding protein BDR-1, Hippocalcin-like protein 1, HLP2, VILIP-3, visinin-like protein 3, VILIP3, ATGP0716-10 µg, ATGP0716-20 µg, ATGP0716-50 µg, ATGP0716-100 µg, ATGP0716-250 µg, ATGP0716-500 µg, ATGP0716-1 mg, ATGP0716-10, ATGP0716-20, ATGP0716-50, ATGP0716-100, ATGP0716-250, ATGP0716-500, ATGP0716-1

Fu J., et al. (2009) J Biol Chem. 284 (40) :27577-86.; ; Spilker C., et al. (2003) Mol Cell Neurosci. 24 (3) :766-78.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 1mM DTT, 10% glycerol

Neuroscience

NP_002140

P37235

Human

MGKQNSKLRP EVLQDLRENT EFTDHELQEW YKGFLKDCPT GHLTVDEFKK IYANFFPYGD ASKFAEHVFR TFDTNGDGTI DFREFIIALS VTSRGKLEQK LKWAFSMYDL DGNGYISRSE MLEIVQAIYK MVSSVMKMPE DESTPEKRTD KIFRQMDTNN DGKLSLEEFI RGAKSDPSIV RLLQCDPSSA SQF