Recombinant humaan HPD-eiwit

4-hydroxyphenylpyruvate dioxygenase isoform 1, also known as HPD, is an Fe-containing enzyme, that catalyzes the second reaction in the catabolism of tyrosine the conversion of 4-hydroxyphenylpyruvate to homogentisate. Existing as a homodimer, HPD uses zinc as a cofactor to catalyze the third step in the conversion of L-phenylalanine to fumarate and acetoacetic acid. Defects in the gene encoding HPD are the cause of tyrosinemia type 3 and hawkinsinuria, both of which are inborn errors of metabolism that are associated with a variety of symptoms, including mental retardation and seizures and hair and urine abnormalities. Recombinant human HPD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

4-hydroxyphenylpyruvate dioxygenase isoform 1, 4-HPPD, 4HPPD, GLOD3, HPPDASE, PPD, 4-hydroxyphenylpyruvic acid oxidase, Glyoxalase domain containing 3

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

47 kDa (413aa) confirmed by MALDI-TOF

HPD, 4-hydroxyphenylpyruvate dioxygenase isoform 1, 4-HPPD, 4HPPD, GLOD3, HPPDASE, PPD, ATGP0755-10 µg, ATGP0755-20 µg, ATGP0755-50 µg, ATGP0755-100 µg, ATGP0755-250 µg, ATGP0755-500 µg, ATGP0755-1 mg, ATGP0755-10, ATGP0755-20, ATGP0755-50, ATGP0755-100, ATGP0755-250, ATGP0755-500, ATGP0755-1

Wada GH., et al. (1975) J Biol Chem J. 250 (17) :6720-6.; ; uetschi u., et al. (1997) Genomics. 44:292-299.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 50 mM NaCl

Metabolism

NP_002141

P32754

Human

MTTYSDKGAK PERGRFLHFH SVTFWVGNAK QAASFYCSKM GFEPLAYRGL ETGSREVVSH VIKQGKIVFV LSSALNPWNK EMGDHLVKHG DGVKDIAFEV EDCDYIVQKA RERGAKIMRE PWVEQDKFGK VKFAVLQTYG DTTHTLVEKM NYIGQFLPGY EAPAFMDPLL PKLPKCSLEM IDHIVGNQPD QEMVSASEWY LKNLQFHRFW SVDDTQVHTE YSSLRSIVVA NYEESIKMPI NEPAPGKKKS QIQEYVDYNG GAGVQHIALK TEDIITAIRH LRERGLEFLS VPSTYYKQLR EKLKTAKIKV KENIDALEEL KILVDYDEKG YLLQIFTKPV QDRPTLFLEV IQRHNHQGFG AGNFNSLFKA FEEEQNLRGN LTNMETNGVV PGM