Recombinant humaan HPRT-eiwit

Hypoxanthine-guanine phosphoribosyltransferase, also known as HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. The enzyme primarily functions to salvage purines from degraded DNA to renewed purine synthesis. In this role, it acts as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP. Recombinant human HPRT1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 15 units/mg and is defined as the amount of enzyme that catalyze the formation of 1 umole of guanosine 5-monophosphate (GMP) per minute from guanine and phosphoribosyl pyrophosphate at pH 7.5 at 37C.

Hypoxanthine-guanine phosphoribosyltransferase, HGPRT, HGPRTase, HPRT

E.coli

Human

His-Tag

Enzyme Activity, SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

Specific activity is > 15unit/mg and is defined as the amount of enzyme that catalyze the formation of 1 umole of guanosine 5-monophosphate (GMP) per minute from guanine and phosphoribosyl pyrophosphate at pH 7.5 at 37C.

26.7 kDa (238aa) confirmed by MALDI-TOF

HPRT, Hypoxanthine-guanine phosphoribosyltransferase, HGPRT, HGPRTase, HPRT, ATGP3466-10 µg, ATGP3466-20 µg, ATGP3466-50 µg, ATGP3466-100 µg, ATGP3466-250 µg, ATGP3466-500 µg, ATGP3466-1 mg, ATGP3466-10, ATGP3466-20, ATGP3466-50, ATGP3466-100, ATGP3466-250, ATGP3466-500, ATGP3466-1

Hladnik U., et al. (2008) Arch Neurol. 65 (9) :1240-3.; ; Sculley DG., et al. (1992) Hum Genet. 90 (3) :195-207191.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing, 20% glycerol

Enzymes & Proteases

NP_000185

P00492

Human

MGSSHHHHHH SSGLVPRGSH MATRSPGVVI SDDEPGYDLD LFCIPNHYAE DLERVFIPHG LIMDRTERLA RDVMKEMGGH HIVALCVLKG GYKFFADLLD YIKALNRNSD RSIPMTVDFI RLKSYCNDQS TGDIKVIGGD DLSTLTGKNV LIVEDIIDTG KTMQTLLSLV RQYNPKMVKV ASLLVKRTPR SVGYKPDFVG FEIPDKFVVG YALDYNEYFR DLNHVCVISE TGKAKYKA