Recombinant humaan HRSP12/RIDA-eiwit

HRSP12, also called ribonuclease uK114, is an endoribonuclease found predominantly in human adult kidney and liver, and which is responsible for inhibiting translation by cleaving mRNA. This protein cleaves phosphodiester bonds only in single-stranded RNA. It may be an important biomarker for heptatic carcinoma. Recombinant human HRSP12 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Reactive intermediate imine deaminase A homolog, 2-iminobutanoate/2-iminopropanoate deaminase, Heat-responsive protein 12, Translational inhibitor p14.5, Translation inhibitor L-PSP ribonuclease, UK114 antigen homolog, P14.5, PSP

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by BCA assay)

> 95% by SDS-PAGE

16.6 kDa (157aa) confirmed by MALDI-TOF

HRSP 12, Ribonuclease uK114, p14.5, PSP, uK114, ATGP0837-10 µg, ATGP0837-20 µg, ATGP0837-50 µg, ATGP0837-100 µg, ATGP0837-250 µg, ATGP0837-500 µg, ATGP0837-1 mg, ATGP0837-10, ATGP0837-20, ATGP0837-50, ATGP0837-100, ATGP0837-250, ATGP0837-500, ATGP0837-1

Schmiedeknecht G., et al. (1996) . Eur J Biochem. 242 (2) :339-51.; ; Manjasetty BA, et al. (2004) . Proteins 54 (4) :797-800

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl, 1mM DTT

Cancer

NP_005827

P52758

Human

MGSSHHHHHH SSGLVPRGSH MSSLIRRVIS TAKAPGAIGP YSQAVLVDRT IYISGQIGMD PSSGQLVSGG VAEEAKQALK NMGEILKAAG CDFTNVVKTT VLLADINDFN TVNEIYKQYF KSNFPARAAY QVAALPKGSR IEIEAVAIQG PLTTASL