Recombinant humaan HS3ST1-eiwit

Heparan sulfate glucosamine 3-O-sulfotransferase 1, also known as HS3ST1, is sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. This protein catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact) . This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site. Recombinant human HS3ST1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Heparan sulfate glucosamine 3-O-sulfotransferase 1, Heparan sulfate glucosamine 3-O-sulfotransferase 1, 3OST, 3OST1

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

36.2 kDa (310aa) confirmed by MALDI-TOF

HS3ST1, Heparan sulfate glucosamine 3-O-sulfotransferase 1, Heparan sulfate glucosamine 3-O-sulfotransferase 1, 3OST, 3OST1, ATGP2320-10 µg, ATGP2320-20 µg, ATGP2320-50 µg, ATGP2320-100 µg, ATGP2320-250 µg, ATGP2320-500 µg, ATGP2320-1 mg, ATGP2320-10, ATGP2320-20, ATGP2320-50, ATGP2320-100, ATGP2320-250, ATGP2320-500, ATGP2320-1

Shworak N.W., et al. (1997) J. Biol. Chem. 272:28008-28019

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 2mM DTT

Signal Transduction

NP_005105

O14792

Human

MGSSHHHHHH SSGLVPRGSH MGSRPAELGQ QELLRKAGTL QDDVRDGVAP NGSAQQLPQT IIIGVRKGGT RALLEMLSLH PDVAAAENEV HFFDWEEHYS HGLGWYLSQM PFSWPHQLTV EKTPAYFTSP KVPERVYSMN PSIRLLLILR DPSERVLSDY TQVFYNHMQK HKPYPSIEEF LVRDGRLNVD YKALNRSLYH VHMQNWLRFF PLRHIHIVDG DRLIRDPFPE IQKVERFLKL SPQINASNFY FNKTKGFYCL RDSGRDRCLH ESKGRAHPQV DPKLLNKLHE YFHEPNKKFF ELVGRTFDWH