Recombinant humaan HSP27-eiwit

Hsp27 (also known as the Estrogen-Regulated 24K protein, and hsp 28) is a member of the mammalian small heat shock protein family. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. Also, Hsp27 is phosphorylated in vivo on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPKAP kinase 2 and the stress-activated protein kinase SAPK2 (p38) . Recombinant human HSP27 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Heat shock protein beta-1, CMT2F, HMN2B, Hsp25, Heat shock protein beta-1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

24.9 kDa (225aa) confirmed by MALDI-TOF

HSP27, Heat shock protein beta-1, CMT2F, HMN2B, Hsp25, Heat shock protein beta-1, ATGP0444-10 µg, ATGP0444-20 µg, ATGP0444-50 µg, ATGP0444-100 µg, ATGP0444-250 µg, ATGP0444-500 µg, ATGP0444-1 mg, ATGP0444-10, ATGP0444-20, ATGP0444-50, ATGP0444-100, ATGP0444-250, ATGP0444-500, ATGP0444-1

Friedman MJ., et al. (2009) J Biol Chem. 284 (41) :27944-51. ; ; Brunet Simioni M., et al. (2009) Oncogene. 28 (37) :3332-44.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1 M NaCl.

Heat Shock Proteins

NP_001531

P04792

Human

MTERRVPFSL LRGPSWDPFR DWYPHSRLFD QAFGLPRLPE EWSQWLGGSS WPGYVRPLPP AAIESPAVAA PAYSRALSRQ LSSGVSEIRH TADRWRVSLD VNHFAPDELT VKTKDGVVEI TGKHEERQDE HGYISRCFTR KYTLPPGVDP TQVSSSLSPE GTLTVEAPMP KLATQSNEIT IPVTFESRAQ LGGPEAAKSD ETAAK